Improved reliability of lymphoma diagnostics via PCR-based clonality testing: F Report of the BIOMED-2 Concerted Action BHM4-CT98-3936
نویسندگان
چکیده
Department of Pathology, UMC St Radboud, Nijmegen, The Netherlands; Department of Immunology, Erasmus MC, University Medical Center Rotterdam, Rotterdam, The Netherlands; Laboratoire d’Hematologie, Hôpital Necker-Enfants Malades, Paris, France; II Medical Department, University Hospital Schleswig-Holstein, Kiel, Germany; Department of Immunology, Southampton General Hospital, Southampton, UK; Department of Hematology, Hospital Universitario de Salamanca, Salamanca, Spain; Department of Molecular Haematology, University of Leeds, Leeds, UK; Department of Haematology, Instituto Portugués de Oncologia, Lisbon, Portugal; Department of Pathology, Hotel-Dieu de Paris, Paris, France; Department of Pathology, Instituto Portugués de Oncologia, Lisbon, Portugal; Department of Pathology, CHU Henri Mondor, Creteil, France; Department of Pathology, University of Wales College of Medicine, Cardif, UK; Molecular Pathology Program, CNIO, Madrid, Spain; Department of Pathology, Luitpoldkrankenhaus, Würzburg, Germany; Department of Pathology, Johann Wolfgang Goethe University Hospital, Frankfurt, Germany; Department of Pathology, Centre Hospitalier Lyon-Sud, PierreBenite, France; Institut für Pathologie, Charité – Universitätsmedizin Berlin, Berlin, Germany; Department of Haematology, Hopital Pitié-Salpétière, Paris, France; Wessex Immunology Service, Molecular Pathology Unit, Southampton University Hospitals, Southampton, UK; Department of Pathology, University Medical Center Groningen, Groningen, The Netherlands; Department of Haematology, Centre Hospitalier Lyon-Sud, Lyon, France and Department of Molecular Biology, Instituto Português de Oncologia, Lisbon, Portugal
منابع مشابه
Pitfalls in TCR gene clonality testing: teaching cases
Clonality testing in T-lymphoproliferations has technically become relatively easy to perform in routine laboratories using standardized multiplex polymerase chain reaction protocols for T-cell receptor (TCR) gene analysis as developed by the BIOMED-2 Concerted Action BMH4-CT98-3936. Expertise with clonality diagnostics and knowledge about the biology of TCR gene recombination are essential for...
متن کامل[Genotypic analysis in primary cutaneous lymphomas using the standardized BIOMED-2 polymerase chain reaction protocols].
The European Biomedicine and Health (BIOMED-2) Concerted Action Project BMH4-CT98-3936 has defined standardized protocols for polymerase chain reaction (PCR) amplification of different loci of the T-cell receptor (TCR) and immunoglobulin (Ig) genes with a view to achieving greater sensitivity and specificity in the assessment of clonality of lymphoid neoplasms. To assess T-cell clonality, analy...
متن کاملMolecular immunoglobulin/T- cell receptor clonality analysis in cutaneous lymphoproliferations. Experience with the BIOMED-2 standardized polymerase chain reaction protocol.
BACKGROUND AND OBJECTIVES Molecular clonality analysis of immunoglobulin (Ig) and T-cell receptor (TCR) genes is a widely used diagnostic tool for discrimination between polyclonal, oligoclonal, and monoclonal lymphoproliferative skin lesions. We studied Ig/TCR clonality in a series of 60 patients with an initial suspicion of (primary) cutaneous B- or T-cell lymphoma (CBCL/CTCL). Clonality of I...
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REFERENCES 1 Gillmore JD, Hawkins PN. Amyloidosis and the respiratory tract. Thorax 1999; 54: 444–451. 2 Capizzi SA, Betancourt E, Prakash UB. Tracheobronchial amyloidosis. Mayo Clin Proc 2000; 75: 1148–1152. 3 Evans PA, Pott C, Groenen PJ, et al. Significantly improved PCRbased clonality testing in B-cell malignancies by use of multiple immunoglobulin gene targets. Report of the BIOMED-2 Conce...
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REFERENCES 1. Lossos IS. Molecular pathogenesis of diffuse large B-cell lymphoma. J Clin Oncol 2005;23:6351-7. 2. Kang YH, Park CJ, Seo EJ, et al. Polymerase chain reaction-based diagnosis of bone marrow involvement in 170 cases of non-Hodgkin lymphoma. Cancer 2002;94:3073-82. 3. Sandberg Y, van Gastel-Mol EJ, Verhaaf B, Lam KH, van Dongen JJ, Langerak AW. BIOMED-2 multiplex immunoglobulin/T-ce...
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